Current projects:

Modification of physico-chemical properties of lung fibroblasts in response to chemical modification of two- and three-dimensional substrates with controlled elasticity

The main goal of the presented project is a performance of comparative analysis of physico-chemical properties of originating from two distinct Interstitial Lung Diseases (ILD) - idiopathic pulmonary fibrosis (IPF) and nonspecific interstitial pneumonia (NSIP) cultured on two- and three-dimensional elastomer (PDMS) substrates with tuned mechanical and chemical properties and defining a set of parameters enabling label-free distinction between them.

Acknowledgment:
This work is financially supported by the National Science Centre under project number UMO-2017/25/B/ST5/00575

 

Finished projects:

Modification of adhesion and proliferation of malignant and non-malignant bladder cancer cells by substrates with combined mechanical, chemical and topographical properties

The main goal of the presented project is to design photosensitive polydimethylsiloxane (PDMS) substrate which, due to appropriate adjusted mechanical (elasticity), chemical (type of polymer coverage) and topographical (size and shape of polymer pattern), allows to control and moderate adhesion and proliferation of bladder cancer cell lines, at different stage of cancer progression.

Articles:

"Elasticity patterns induced by phase-separation in polymer blend films" J. Raczkowska, S. Prauzner-Bechcicki, P. Dąbczyński, R. Szydlak, Thin Solid Films 624 (2017), 181-186

"Patterning of cancerous cells driven by a combined modification of mechanical and chemical properties of the substrate" J. Raczkowska, K. Awsiuk, S. Prauzner-Bechcicki, J. Pabijan, J. Zemła, A. Budkowski, M. Lekka, European Polymer Journal (2017) in press

"Precise positioning of cancerous cells on PDMS substrates with gradients of elasticity" J. Raczkowska, S. Prauzner-Bechcicki, Biomedical Microdevices 18 (2016), 1-8

"Physico-chemical properties of PDMS surfaces suitable as substrates for cell cultures" J. Raczkowska, S. Prauzner-Bechcicki, J. Lukes, J. Sepitka, A. Bernasik, K. Awsiuk, C. Paluszkiewicz, J. Pabijan, M. Lekka, A. Budkowski, Applied Surface Science 389 (2016), 247–254

Acknowledgment:
This work is financially supported by the National Science Centre under project number DEC-2013/09/D/ST5/03859