Current projects:

Impact of polymer tacticity on adsorption, conformation and orientation of proteins on surface of thin polymer films

The main goal of the presented project is to investigate the influence of polymer tacticity on the adsorption of proteins to thin polymer films. For potential biomedical applications not only the amount of adsorbed molecules, but also their orientation and conformation play a crucial role as they affect strongly the bioactivity of proteins. Therefore, the effect of polymer tacticity on conformation and orientation of adsorbed macromolecules will be investigated.

Acknowledgment:
This work is financially supported by the National Science Centre under project number UMO-2016/21/D/ST5/01633

 

Finished projects:

Protein immobilisation to film surfaces and micro-patterns of conjugated polymer in the aspect of biosensors applications

The main goal of the project is to analyse protein immobilisation (adsorption and specific binding) to film surfaces and micro-patterns of conjugated polymers. The conjugated polymers group includes poly(3-alkylthiophenes) which were chosen because they are widely applied in plastic electronics. The proposed researches aim at analysis of potential application and then at selection of poly(3-alkylthiophenes) with optimal properties to construct biosensors. Two protein systems will be examined: streptavidin-biotin as well as γ-globulin and its antigen. These systems were chosen due to their common application for functionalization of bioactive biosensors surfaces. Researches will focus on analysis of protein: streptavidin, γ-globulin and bovine serum albumin (BSA) adsorption to film surfaces and micropatterns of conjugated polymers. Moreover, the biological activity of adsorbed proteins will be checked by testing their ability to bind specifically biotin or antigen in the case of adsorbed molecules of streptavidin and γ-globulin, respectively.

Articles:

"Orientation and biorecognition of immunoglobulin adsorbed on spin-cast poly(3-alkylthiophenes): impact of polymer film crystallinity" K. Awsiuk, A. Budkowski, P. Petrou, M. M. Marzec, M. Biernat, T. Jaworska-Gołąb, J. Rysz, Colloids and Surfaces B: Biointerfaces 148 (2016), 278-286

"Effects of polythiophene surface structure on adsorption and conformation of bovine serum albumin: A multivariate and multitechnique study" K. Awsiuk, A. Budkowski, M.M. Marzec, P. Petrou, J. Rysz, A. Bernasik, Langmuir 30 (2014), 13925–13933

Acknowledgment:
This work is supported by the Polish National Science Center (NCN) under Grant UMO-2011/03/N/ST5/04764